FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n02_DS2099-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n02_DS2099-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18909835
Sequences flagged as poor quality0
Sequence length37
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT2871661.5186065875244283Illumina Single End PCR Primer 1 (100% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG354910.18768540286046917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGAT372200.026.36175531
AGTGTAG386000.025.8335229
GAGTGTA389000.025.40712528
AGAGTGT397900.024.92082627
GTGTAGA403800.024.66716430
AAGAGTG424750.023.54611826
GTAGGGA459300.022.44943419
TGTAGGG460550.022.3659518
CGTGTAG467750.022.24116516
GTGTAGG468050.022.06124917
AAAGAGT461200.021.98439625
TCGTGTA488900.021.8056415
GTCGTGT489300.021.77836414
TAGGGAA478950.021.71819120
GCGTCGT507400.021.31317112
CGTCGTG511150.021.15675513
GGGAAAG480900.021.09409122
AGCGTCG515900.021.06095511
GAGCGTC530300.020.50643210
GGAAAGA513700.020.24785623
Produced by FastQC (version 0.11.5)