FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n02_DS2094-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n02_DS2094-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16048545
Sequences flagged as poor quality0
Sequence length37
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT1190630.7418928008738487Illumina Single End PCR Primer 1 (100% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG362160.22566531732315923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG168400.024.517229
GAGTGTA175000.023.4246528
TGTAGAT177600.022.89753731
GTGTAGA181850.022.652330
AGAGTGT182350.022.58281327
TGTAGGG203350.020.75739718
CGTGTAG207100.020.71178216
GTAGGGA202900.020.68016419
GTCGTGT213050.020.65775114
AAGAGTG203000.020.5070726
GTGTAGG209050.020.31855417
TCGTGTA217300.020.23932815
GCGTCGT221050.020.1977312
AGCGTCG222800.020.08076911
TAGGGAA216250.019.61761320
CGTCGTG229100.019.54198313
GAGCGTC235200.018.9952510
AGAGCGT237150.018.935349
AAGAGCG239500.018.8915778
AAAGAGT232700.018.09651825
Produced by FastQC (version 0.11.5)