FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n02_DS2093-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n02_DS2093-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19686759
Sequences flagged as poor quality0
Sequence length37
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT2098871.0661328256215257Illumina Single End PCR Primer 1 (100% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG515610.2619070005377726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG285950.025.48927129
GAGTGTA290500.025.05301528
TGTAGAT287250.025.00621231
GTGTAGA296550.024.6245230
AGAGTGT298550.024.44009827
AAGAGTG327400.022.54726
CGTGTAG350800.021.42813316
TGTAGGG349700.021.29507818
GTAGGGA350650.021.2540419
GTCGTGT363400.021.2227714
GTGTAGG351850.021.17451717
TCGTGTA366600.021.04180115
GCGTCGT374400.020.84777612
AGCGTCG377600.020.74910511
TAGGGAA363600.020.68326620
CGTCGTG377700.020.62858413
AAAGAGT370200.020.20861825
GAGCGTC390700.020.0291310
GCACACG28450.019.9420918
AAGAGCG396200.019.8860138
Produced by FastQC (version 0.11.5)