FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n02_DS2091-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n02_DS2091-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19979873
Sequences flagged as poor quality0
Sequence length37
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT2758921.3808496180130876Illumina Single End PCR Primer 1 (100% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG360350.026.82013929
GAGTGTA359050.026.78370528
AGAGTGT369750.026.101127
GTGTAGA370550.026.04782330
TGTAGAT368550.025.90724231
AAGAGTG390100.025.00172226
GTAGGGA451100.023.58269719
GGGAAAG440650.023.57324822
TGTAGGG454600.023.3645618
CGTGTAG462250.023.27086316
GTGTAGG458750.023.26907517
GTCGTGT480000.022.8401514
TAGGGAA467300.022.7837420
AAAGAGT438450.022.72551225
CGTCGTG493550.022.58076113
GGAAAGA469950.022.4923623
GCGTCGT496100.022.47088412
GAAAGAG465800.022.32973124
AGCGTCG501350.022.32215511
TCGTGTA495700.022.21978615
Produced by FastQC (version 0.11.5)