FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n02_DS2090-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n02_DS2090-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21825675
Sequences flagged as poor quality0
Sequence length37
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT3699681.695104504213501Illumina Single End PCR Primer 1 (100% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG710280.32543323402368995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG481150.026.6637929
TGTAGAT476150.026.5881531
GAGTGTA485150.026.33865428
GTGTAGA490000.026.13398730
AGAGTGT499600.025.64873527
AAGAGTG522950.024.68160826
AAAGAGT584600.022.30436925
GTAGGGA600950.021.72338519
TGTAGGG603900.021.65681318
TAGGGAA609400.021.56132320
CGTGTAG610750.021.54668616
GGGAAAG607550.021.44245122
GTGTAGG610900.021.42725617
GTCGTGT637100.021.21049714
TCGTGTA638600.021.1267915
GGAAAGA634650.020.94173423
GCGTCGT651800.020.87265212
AGCGTCG659250.020.70487611
GAAAGAG633600.020.69958724
CGTCGTG661400.020.60475713
Produced by FastQC (version 0.11.5)