FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n02_DS2088-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n02_DS2088-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46353939
Sequences flagged as poor quality0
Sequence length37
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT4410220.9514229200672677Illumina Single End PCR Primer 1 (100% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1266640.27325401623365814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG589900.025.69980429
GAGTGTA608400.024.87516428
TGTAGAT625800.023.8803331
AGAGTGT642150.023.65253327
GTGTAGA644400.023.52781730
AAGAGTG691950.022.20809226
GCACACG61150.019.62047218
TGTAGGG794200.019.5628818
GTAGGGA796050.019.53028919
CGTGTAG803900.019.5264116
GTGTAGG808700.019.27036917
GTCGTGT840200.019.1498114
TAGGGAA823150.019.07849520
AAAGAGT818150.019.01384225
TCGTGTA850150.018.95471615
GCGTCGT862350.018.84323712
AGCGTCG869800.018.73350711
CGTCGTG880950.018.47874513
GGGAAAG849450.018.233422
GAGCGTC908200.017.9667810
Produced by FastQC (version 0.11.5)