FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n02_DS2087-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n02_DS2087-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22285558
Sequences flagged as poor quality0
Sequence length37
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG441820.19825395442196245No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT339770.15246196662430442Illumina Single End PCR Primer 1 (100% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG80850.018.6181611
GCGTCGT80200.018.537112
GCACACG28150.018.22723618
GTCGTGT80300.018.20504414
CGTGTAG75850.017.92399216
AGCACAC46350.016.6891317
TGTAGGG77000.016.52812818
GTGTAGG79900.016.43310217
AAGAGCG94650.016.1311158
AGTGTAG74500.016.1012829
TCGTGTA93600.016.09841315
AGAGCAC57800.016.09222415
GTAGGGA80050.016.07187519
CGTCGTG95250.016.03126713
GAGCACA56650.015.65261216
CGGGGTG14300.015.4979661
AGAGCGT99350.015.430479
CACACGT25100.015.37713319
GAGCGTC99500.015.28402110
CGGGGAG12450.014.6888361
Produced by FastQC (version 0.11.5)