FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16219383
Sequences flagged as poor quality0
Sequence length37
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGA13451558.293502903285532TruSeq Adapter, Index 2 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG533840.3291370578030003No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGA505090.3114113527006545TruSeq Adapter, Index 14 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGG486150.29973396645236133TruSeq Adapter, Index 14 (97% over 35bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCG343430.21174048359299488TruSeq Adapter, Index 14 (97% over 36bp)
GAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAG216460.13345760439839172TruSeq Adapter, Index 14 (96% over 30bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAT211510.1304057003894661TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCACTCACAGA173130.10674265476066505TruSeq Adapter, Index 13 (97% over 35bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACAGAT169400.10444293719434335TruSeq Adapter, Index 2 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2295900.024.392622
ATCGGAA2304850.024.2646773
AGATCGG2350550.023.9974581
AGTCGGA54500.023.9924161
TCGGAAG2357200.023.6704224
CCAGTCA2141800.023.41154927
TCCAGTC2153400.023.3782826
TCACAGA1926200.023.2566131
CAGTCAC2152850.023.21717828
CGGAAGA2407050.023.1740575
GTCACAG1899000.023.14993130
ACTCCAG2229200.023.09929724
AGTCACA1951550.023.09028429
CTCCAGT2194450.023.07444625
GAACTCC2340900.023.01124422
AACTCCA2330250.022.9049123
AAGAGCA2453250.022.7000268
GAAGAGC2455250.022.6651957
TGAACTC2408350.022.65443621
AGAGCAC2448350.022.6484179
Produced by FastQC (version 0.11.5)