FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n01_DS2154-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n01_DS2154-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18837397
Sequences flagged as poor quality0
Sequence length37
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGT1460190.775154868796363TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT196050.025.04607831
GTCACGG196450.024.90033530
AGTCACG198750.024.79934729
CAGTCAC206400.023.93275328
TCCAGTC211700.023.45060326
CCAGTCA211100.023.40712227
GAACTCC227500.022.18303522
ACTCCAG232350.021.56656524
GCACACG236400.021.54566812
ACACGTC237400.021.5397914
CACACGT238150.021.42639413
AGATCGG237500.021.4154051
ACGTCTG239650.021.27287916
CTCCAGT236800.021.10237125
CACGTCT242950.021.0349715
CGTCTGA245400.020.75443617
GATCGGA244950.020.7176232
AGCACAC246900.020.67977311
GTCTGAA247500.020.60338818
TGAACTC246400.020.5884321
Produced by FastQC (version 0.11.5)