FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n01_DS2153-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n01_DS2153-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20825621
Sequences flagged as poor quality0
Sequence length37
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG964350.4630594208931393TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAG132700.024.13130431
GTCACGA134700.023.85355430
AGTCACG137150.023.5178529
CAGTCAC160050.020.23038328
CCAGTCA163050.019.93411327
TCCAGTC167900.019.75524126
GAACTCC182900.019.61808422
ACTCCAG183650.018.93874524
GCACACG197700.018.51102412
ACACGTC198100.018.47360414
GATCGGA202350.018.4529762
CACACGT198700.018.41782213
AGATCGG204250.018.2597831
ACGTCTG200400.018.24611316
CTCCAGT191300.017.88971525
CACGTCT206850.017.72212415
AGCACAC207500.017.7191111
GAGCACA210700.017.60478810
TGAACTC206800.017.56816321
CTGAACT209700.017.36216520
Produced by FastQC (version 0.11.5)