FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n01_DS2152-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n01_DS2152-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21226848
Sequences flagged as poor quality0
Sequence length37
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGT2442061.1504581367897861TruSeq Adapter, Index 12 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGT318050.024.888131
GTCACCG318050.024.83449430
CAGTCAC323400.024.76393928
TCCAGTC328800.024.63995626
AGTCACC321700.024.63462829
CCAGTCA328100.024.53190827
GAACTCC356800.023.20154822
ACTCCAG357050.023.03336724
CTCCAGT360900.022.60727725
ACACGTC378450.022.12486514
ACGTCTG378800.022.07163416
TGAACTC377800.022.04317321
GCACACG379350.022.035612
CACACGT380150.022.00553913
CACGTCT382600.021.8807815
CGTCTGA385000.021.71950717
CTGAACT386650.021.58272420
GTCTGAA388150.021.53126318
TCTGAAC390700.021.43437419
AGCACAC390700.021.39957611
Produced by FastQC (version 0.11.5)