FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n01_DS2151-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n01_DS2151-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19919236
Sequences flagged as poor quality0
Sequence length37
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTT1724830.8659117247267917TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG222250.024.99465629
GTCACGT223500.024.73003430
CAGTCAC233150.023.99897428
TCACGTT230950.023.91886731
CCAGTCA236200.023.79401427
TCCAGTC238750.023.59830926
GAACTCC263850.021.9525922
ACTCCAG267700.021.49791724
GCACACG274900.021.24581512
ACGTCTG276150.021.16621816
ACACGTC276600.021.1261814
CACACGT277700.021.07609213
CTCCAGT272100.020.96800425
CACGTCT278950.020.96487415
CGTCTGA282250.020.6642717
TGAACTC282500.020.65695821
AGCACAC285750.020.46633111
GTCTGAA285900.020.44382718
AGATCGG286400.020.4220351
GAGCACA289550.020.21945210
Produced by FastQC (version 0.11.5)