FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n01_DS2150-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n01_DS2150-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18206717
Sequences flagged as poor quality0
Sequence length37
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG3915402.1505249958023733TruSeq Adapter, Index 15 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGTG476900.026.6799431
GTCACGT500600.025.50661530
AGTCACG507500.025.20563729
CAGTCAC561250.022.84972828
CCAGTCA565650.022.68824227
TCCAGTC579450.022.45016526
AGTCGGA37050.021.8822821
GAACTCC639450.021.34471522
ACTCCAG629250.021.16359124
CTCCAGT634650.020.72464225
AACTCCA668900.020.2844723
TGAACTC688150.019.98732421
CTGAACT703450.019.64954820
ACGTCTG729450.019.03287716
GTCGGAA39450.019.016832
GTCTGAA729300.019.00195118
ACACGTC733150.018.9812214
CGTCTGA733200.018.93258717
TCTGAAC733150.018.8937119
CACGTCT736650.018.8931415
Produced by FastQC (version 0.11.5)