FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n01_DS2149-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n01_DS2149-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21920673
Sequences flagged as poor quality0
Sequence length37
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG1201370.5480534288340508TruSeq Adapter, Index 15 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGTG154450.025.10841431
AGTCACG162550.024.13376429
GTCACGT163050.023.89815330
CAGTCAC187000.021.10263428
GAACTCC203000.020.75254222
TCCAGTC194150.020.66068826
CCAGTCA192250.020.63107327
ACTCCAG206600.019.8732824
GCACACG222000.019.46616712
ACACGTC223300.019.36663614
CACACGT224550.019.2174113
GATCGGA226250.019.1485942
ACGTCTG225450.019.08560816
CTCCAGT214450.018.94344125
AGATCGG229250.018.8996071
GAGCACA231900.018.76243810
AGCACAC230800.018.74427611
CACGTCT231900.018.6617915
TGAACTC230850.018.51078221
GTCTGAA232750.018.44624518
Produced by FastQC (version 0.11.5)