FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n01_DS2148-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n01_DS2148-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28360694
Sequences flagged as poor quality0
Sequence length37
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTC2584540.9113105624284088TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGTC324800.026.32266831
GTCACGT345150.024.89189130
AGTCACG345550.024.85859329
CAGTCAC390400.022.1496828
CCAGTCA397050.021.82543227
TCCAGTC404850.021.66527426
GAACTCC443850.020.76731122
ACTCCAG440900.020.41058724
CTCCAGT446800.019.89476625
TGAACTC489500.019.01106821
CTGAACT497600.018.73586820
ACGTCTG500300.018.71893316
ACACGTC504050.018.6781714
GCACACG509050.018.49480612
CACGTCT509100.018.46241415
GTCTGAA507150.018.44418318
CACACGT510900.018.4307713
AACTCCA503500.018.28236623
TCTGAAC514650.018.17840819
CGTCTGA517750.018.08752817
Produced by FastQC (version 0.11.5)