FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n01_DS2147-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n01_DS2147-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19720002
Sequences flagged as poor quality0
Sequence length37
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTA10862605.50841729123557TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAG325550.1650861901535304TruSeq Adapter, Index 3 (97% over 37bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT222990.11307808183792273TruSeq Adapter, Index 1 (97% over 35bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT212070.10754055704456825TruSeq Adapter, Index 1 (97% over 35bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAG207960.10545637875695955TruSeq Adapter, Index 18 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGTA1261500.027.58747131
GTCACGT1315450.026.4619330
AGTCACG1322650.026.39053329
CAGTCAC1342350.026.06327628
CCAGTCA1345300.026.01392427
TCCAGTC1350150.025.96869526
ACTCCAG1404750.025.15794824
CTCCAGT1404900.025.0835525
GAACTCC1427800.024.8701322
AACTCCA1434050.024.78335423
TGAACTC1474150.024.17017721
CTGAACT1499950.023.80713720
TCTGAAC1519700.023.54057719
GTCTGAA1519800.023.53698718
CGTCTGA1525250.023.45904417
ACGTCTG1527450.023.42298316
AGATCGG1537200.023.3044221
CACGTCT1541100.023.26083615
ACACGTC1544650.023.24249814
CACACGT1548750.023.1570113
Produced by FastQC (version 0.11.5)