FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n01_DS2146-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n01_DS2146-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16762864
Sequences flagged as poor quality0
Sequence length37
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT8282694.941094791438981TruSeq Adapter, Index 8 (97% over 36bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG208060.1241196015191676TruSeq Adapter, Index 2 (97% over 35bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG173630.10358015193585059TruSeq Adapter, Index 2 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACAGT966400.027.52385131
AGTCGGA52100.025.3499551
GTCACAG1107400.024.0963530
AGTCACA1148250.023.30794129
CCAGTCA1155700.023.30387727
CAGTCAC1154400.023.29252628
TCCAGTC1187900.023.0257926
GTCGGAA53800.022.7319342
ACTCCAG1289400.021.77580324
GAACTCC1332900.021.66168222
CTCCAGT1282700.021.56812125
AACTCCA1342350.021.34291323
TGAACTC1407150.020.71804821
CTGAACT1439200.020.32021120
GATCGGA1492800.019.9818342
AGATCGG1497800.019.9208151
ACACGTC1482500.019.89754314
ACGTCTG1479100.019.89193316
GTCTGAA1477100.019.86073918
CGTCTGA1482700.019.83485817
Produced by FastQC (version 0.11.5)