FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n01_DS2114-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n01_DS2114-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13023302
Sequences flagged as poor quality0
Sequence length37
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT638330.4901445117374994TruSeq Adapter, Index 12 (100% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACC95850.022.15388929
CAGTCAC98050.021.75165728
GAACTCC101050.021.3972422
CCAGTCA100050.021.3167627
GTCACCT99550.021.09694530
TCCAGTC101700.021.03187626
ACTCCAG104950.020.46918924
GCACACG108550.020.2907212
ACACGTC109450.020.10955614
CACACGT110050.019.98590913
ACGTCTG111550.019.6476116
GATCGGA113100.019.5024382
AGCACAC113900.019.4329811
CTCCAGT110850.019.33776725
AGATCGG114050.019.3275811
CACGTCT114000.019.29333915
GAGCACA115000.019.2743510
CTGAACT115650.018.99083520
GTCTGAA116650.018.80145818
TGAACTC117000.018.7849621
Produced by FastQC (version 0.11.5)