FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n01_DS2112-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n01_DS2112-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17948583
Sequences flagged as poor quality0
Sequence length37
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC3522161.9623610398659328TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGC421300.027.2500931
GTCACGG451050.025.54209530
AGTCACG466400.024.7812229
CAGTCAC512950.022.6350728
CCAGTCA517250.022.46175827
TCCAGTC531700.022.19237726
AGTCGGA29750.021.5200981
GAACTCC608450.020.68198822
ACTCCAG591750.020.57942224
CTCCAGT594700.020.10464325
AACTCCA635450.019.68126323
TGAACTC656100.019.35238621
CTGAACT671600.019.00729420
GTCTGAA685550.018.68156418
ACACGTC684850.018.6769114
ACGTCTG684650.018.6755216
CACGTCT688250.018.5845915
CGTCTGA691250.018.50285317
TCTGAAC692700.018.46635819
CACACGT693050.018.43356513
Produced by FastQC (version 0.11.5)