FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n01_DS2104-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n01_DS2104-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26378991
Sequences flagged as poor quality0
Sequence length37
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG3328521.2618071707139973TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACTAG402150.026.29712731
CAGTCAC422100.025.22309328
AGTCACT427300.024.90163429
CCAGTCA428700.024.82392727
TCCAGTC433650.024.60847926
GTCACTA449700.023.67504530
GAACTCC471200.022.92373322
ACTCCAG475000.022.6196124
CTCCAGT481800.022.2392425
TGAACTC503850.021.56745521
CTGAACT517650.021.05536820
AACTCCA516850.020.99499123
ACGTCTG523350.020.79717816
CGTCTGA524200.020.79227617
GTCTGAA525100.020.75958818
TCTGAAC527600.020.66709919
ACACGTC531050.020.54232814
CACGTCT531250.020.53751415
CACACGT537350.020.26979313
GCACACG540900.020.1397412
Produced by FastQC (version 0.11.5)