FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n01_DS2103-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n01_DS2103-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23545720
Sequences flagged as poor quality0
Sequence length37
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT3750381.59280752510435TruSeq Adapter, Index 9 (100% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAT440600.027.4882431
GTCACGA454400.026.71482730
AGTCACG461950.026.48287629
CAGTCAC499000.024.5911128
CCAGTCA503900.024.36423127
GAACTCC515700.024.31467422
TCCAGTC511750.024.04198626
ACTCCAG520850.023.8838124
CTCCAGT538600.022.97007825
TGAACTC561400.022.4927421
AACTCCA562650.022.35737423
CTGAACT580850.021.79559920
TCTGAAC611550.020.77241719
GTCTGAA613550.020.7021818
ACGTCTG616200.020.64672716
ACACGTC623400.020.47544314
CGTCTGA627550.020.27491417
CACGTCT629250.020.26291715
CACACGT633300.020.13088613
GCACACG638700.019.9437812
Produced by FastQC (version 0.11.5)