FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n01_DS2100-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n01_DS2100-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27456434
Sequences flagged as poor quality0
Sequence length37
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT5353311.9497470064757862TruSeq Adapter, Index 8 (100% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACACT623700.027.90288531
GTCACAC630000.027.62626630
CAGTCAC654100.026.77426328
CCAGTCA657200.026.66433527
TCCAGTC662400.026.5181926
AGTCACA659750.026.4838929
ACTCCAG704350.025.28869424
CTCCAGT706150.025.07497625
GAACTCC718600.024.9511422
AACTCCA759950.023.62818323
TGAACTC766450.023.47835521
CTGAACT782800.023.04935520
TCTGAAC800650.022.56839219
GTCTGAA800800.022.56033718
CGTCTGA801750.022.54133617
ACGTCTG802200.022.52556816
CACGTCT811050.022.29888315
ACACGTC813700.022.24340414
CACACGT817650.022.12836613
GCACACG823350.021.95077912
Produced by FastQC (version 0.11.5)