FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n01_DS2099-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n01_DS2099-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18909835
Sequences flagged as poor quality0
Sequence length37
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG3231511.7089043875845558TruSeq Adapter, Index 7 (100% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCAG391800.026.37876931
AGTCACC407450.025.41495529
CAGTCAC430850.024.21091328
GTCACCA431600.024.06828730
CCAGTCA434150.023.99105627
TCCAGTC439900.023.81135726
GAACTCC456500.023.47515922
ACTCCAG452500.023.43262524
CTCCAGT459700.022.95097725
AACTCCA488000.021.97891423
TGAACTC495050.021.81619521
CTGAACT505800.021.40768420
ACGTCTG517850.020.98536716
GTCTGAA519450.020.85712818
ACACGTC522650.020.78374114
CACGTCT526550.020.63863415
CACACGT525450.020.6375913
TCTGAAC527400.020.5985119
GCACACG527550.020.55848712
CGTCTGA527850.020.55457917
Produced by FastQC (version 0.11.5)