FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n01_DS2094-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n01_DS2094-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16048545
Sequences flagged as poor quality0
Sequence length37
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC1334960.831826187358418TruSeq Adapter, Index 6 (100% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCC161350.026.44606231
GTCACGC168550.025.3346730
AGTCACG176400.024.40055529
CAGTCAC198950.021.70498828
CCAGTCA202450.021.41389827
GAACTCC211950.021.38281822
TCCAGTC207050.021.16272526
ACTCCAG215050.020.75745624
CTCCAGT224400.019.70606825
TGAACTC239600.019.06401421
GCACACG242350.019.00817112
ACACGTC242350.018.95700614
CACACGT243650.018.83677913
AACTCCA241000.018.82464223
CTGAACT243850.018.79531720
GATCGGA245300.018.7606812
ACGTCTG244150.018.7537616
AGATCGG246450.018.6554321
AGCACAC249700.018.4489511
GAGCACA252800.018.2721110
Produced by FastQC (version 0.11.5)