FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n01_DS2093-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n01_DS2093-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19686759
Sequences flagged as poor quality0
Sequence length37
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA2329631.1833486659739167TruSeq Adapter, Index 5 (100% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACACA297200.025.49711831
GTCACAC297850.025.35821330
CAGTCAC340850.022.31375328
AGTCACA341250.022.2194729
CCAGTCA345950.021.9534427
TCCAGTC353750.021.69721626
GAACTCC369700.021.53254922
ACTCCAG372350.021.02547624
CTCCAGT380400.020.38903225
TGAACTC409500.019.59873221
ACACGTC414600.019.56405814
AACTCCA407800.019.54740723
GATCGGA417100.019.4952072
GCACACG417800.019.41050512
AGATCGG419450.019.4055921
CACACGT419200.019.33458513
ACGTCTG420000.019.25711316
CTGAACT418400.019.25592820
CACGTCT423500.019.13461115
AGCACAC426850.019.03561811
Produced by FastQC (version 0.11.5)