FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n01_DS2091-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n01_DS2091-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19979873
Sequences flagged as poor quality0
Sequence length37
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA3009411.5062207852872738TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCAC381100.026.46051228
AGTCACT381850.026.31112129
TCACTGA371800.026.29695131
CCAGTCA385100.026.23792627
TCCAGTC387400.026.2101826
GTCACTG388200.025.90469230
ACTCCAG408300.025.11908124
GAACTCC415600.024.92028222
CTCCAGT412600.024.73333425
AACTCCA443250.023.39722323
TGAACTC445550.023.39472221
CTGAACT457650.022.86084720
CGTCTGA462750.022.72947917
ACGTCTG463650.022.65917816
GTCTGAA465050.022.54040718
TCTGAAC467300.022.4517819
ACACGTC469300.022.44583314
CACGTCT469050.022.42475115
CACACGT472700.022.2614313
GCACACG474950.022.1592912
Produced by FastQC (version 0.11.5)