FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n01_DS2090-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n01_DS2090-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21825675
Sequences flagged as poor quality0
Sequence length37
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA4122171.8886792733787154TruSeq Adapter, Index 3 (100% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACTTA516950.025.90214531
GTCACTT535500.024.94120430
AGTCACT539700.024.7643429
CAGTCAC582650.023.00800328
CCAGTCA588500.022.77660827
TCCAGTC600150.022.54624626
ACTCCAG624150.021.99466924
GAACTCC644750.021.74147422
CTCCAGT638750.021.32935725
AACTCCA674950.020.7388223
AGTCGGA33250.020.3729231
TGAACTC701100.020.1952121
CTGAACT716800.019.7831520
ACGTCTG734350.019.35121316
ACACGTC736000.019.35008414
GCACACG737350.019.29157612
GTCTGAA738400.019.25692218
CACACGT740200.019.21306813
AGATCGG749550.019.1502291
GATCGGA750650.019.1457612
Produced by FastQC (version 0.11.5)