FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n01_DS2088-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n01_DS2088-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46353939
Sequences flagged as poor quality0
Sequence length37
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA4940691.065861954040195TruSeq Adapter, Index 2 (100% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGA627450.025.20654331
GTCACCG646000.024.51872330
AGTCACC660750.024.03941729
CAGTCAC722700.022.0366628
CCAGTCA731150.021.83704827
TCCAGTC748700.021.55082726
GAACTCC833500.020.30478122
ACTCCAG835550.019.75781824
CTCCAGT850400.019.1558125
TGAACTC930850.018.39773221
AACTCCA923100.018.28857823
CTGAACT957200.017.95280620
AGTCGGA54350.017.8543131
GCACACG983050.017.54922512
ACACGTC984500.017.54221314
ACGTCTG984500.017.51542916
CACACGT988950.017.44448913
GTCTGAA990900.017.37978418
GATCGGA1001400.017.3313712
CACGTCT999400.017.27290315
Produced by FastQC (version 0.11.5)