FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n01_DS2087-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n01_DS2087-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22285558
Sequences flagged as poor quality0
Sequence length37
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC373990.16781720251294582TruSeq Adapter, Index 1 (100% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG99650.018.8522612
ACACGTC99850.018.70579314
CAGTCAC93800.018.67231228
AGTCACA93100.018.62957229
CACACGT100950.018.47125613
AGATCGG102250.018.374411
GAACTCC100350.018.27203622
GATCGGA103000.018.2694472
ACGTCTG103450.018.06982416
TCCAGTC98600.017.99899126
CCAGTCA98250.017.88957627
AGCACAC107000.017.62981811
GTCACAT72150.017.48680930
GAGCACA110300.017.32736210
CACGTCT109700.017.05445115
ACTCCAG107150.016.90993324
GTCTGAA114500.016.42006518
AGAGCAC116200.016.3136679
TCGGAAG119200.015.96867754
TCACATC79250.015.86149231
Produced by FastQC (version 0.11.5)