FastQCFastQC Report
Fri 19 Feb 2021
H3MCHDRXY_n01_GG150.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3MCHDRXY_n01_GG150.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70813721
Sequences flagged as poor quality0
Sequence length101
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC32388784.573800040814124TruSeq Adapter, Index 22 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCGCGTATGC6144750.8677343759410695TruSeq Adapter, Index 22 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGGATGC1536330.21695371720404297TruSeq Adapter, Index 22 (97% over 45bp)

[OK]Adapter Content

Adapter graph