FastQCFastQC Report
Fri 19 Feb 2021
H3MCHDRXY_n01_GG149.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3MCHDRXY_n01_GG149.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63636276
Sequences flagged as poor quality0
Sequence length101
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGC21883423.438827878614393TruSeq Adapter, Index 21 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCGCGTATGC4929590.7746509239478439TruSeq Adapter, Index 21 (97% over 42bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)