FastQCFastQC Report
Fri 19 Feb 2021
H3MCHDRXY_n01_GG148.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3MCHDRXY_n01_GG148.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56976385
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGC17576433.0848622635500655TruSeq Adapter, Index 20 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGTATGC4137300.726142945011341TruSeq Adapter, Index 20 (97% over 42bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)