FastQCFastQC Report
Fri 19 Feb 2021
H3MCHDRXY_n01_GG147.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3MCHDRXY_n01_GG147.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70068445
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC11711141.671385742897534TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAGATCTCGTATGC6799060.9703454957506192TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCGCGTATGC2311880.3299459549872985TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGTATGC1564760.2233187849394974TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAGATCGCGTATGC1384740.19762676337401236TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAGATCTGGTATGC1066590.15222116032402316TruSeq Adapter, Index 20 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)