FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l02n02 root 2.34200000009427.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l02n02 root 2.34200000009427.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19457105
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACACA836450.014.42320645
GTCTTAG4750.014.1981161
TATACAC1220800.014.165956545
TCTGACG94950.014.01773645
TACACAT568750.013.48377345
GTAACAC4850.013.4511623
ACACATC408100.013.37070545
TACACCG53350.013.3272695
CTATACT5600.012.8573194
CACATCT291500.012.618155545
TATACTG5400.012.5001715
TAGGACA11000.012.4774444
TATACCG10500.012.0001655
CTTATAA9050.011.924645
TTAGTAC4350.011.8943893
TCTATAC7850.011.4628843
TCTAGAC5300.011.4601813
TTATACA1635250.011.38681345
CTAGACT4750.011.3685774
CTAGGAC6750.011.3312733