FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l02n02 heat recovery 4h 2.342000000092f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l02n02 heat recovery 4h 2.342000000092f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14320515
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT13200.021.45924245
TAGACGG10400.020.3358755
GTACCGT15100.017.7312096
CCTAGAC26700.016.5136593
TATACAC319050.016.07957845
GACGGTC10050.015.89578157
GCCCTAG13050.015.8479221
TGCGTCG11800.015.06796625
TACCGTG20250.014.8891327
GTCTAGG28700.014.6472041
ATACACA211000.014.45823145
AGTACCG23500.014.169755
GTCTATG31550.014.107851
CCGTGCG9950.014.0213099
ACTGTCC14050.013.9326128
GCGTCGT12750.013.770226526
CTAGACC21900.013.6639384
GACTGTG8650.013.5262337
TATACTG40650.013.4497455
TACACAT136100.013.41268345
Produced by FastQC (version 0.11.2)