FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l02n02 heat 4h 3.342000000093bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l02n02 heat 4h 3.342000000093bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11309375
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG9250.022.8640215
GTACCGT14500.016.7580036
GTCTATG23900.016.3670371
GACGGTC11000.016.3640467
CCTAGAC22000.016.1552053
GACTGTG6350.015.5909427
GACAGTG10700.015.3508537
GTCTAGG22900.014.9219931
AGTACCG19350.014.8831725
GCCCTAG11400.014.7902361
TATACAC139000.014.31544345
TTAGGAC20350.014.259473
GTATAAG42800.014.0244621
GGACTGC20100.013.6562126
GTCTAAG20850.013.5857361
ATAGACG13900.013.2728924
GTGTATG32200.013.1954621
CTAGACC14550.013.1438454
TAGCCCG12900.013.08375118
GTCTTAG19250.013.07994651
Produced by FastQC (version 0.11.2)