FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l02n02 heat 30 min 3.34200000009335.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l02n02 heat 30 min 3.34200000009335.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13965125
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG11550.020.25975
CCTAGAC23500.017.1343963
GTCTAGG27150.016.0633811
GTACCGT16650.015.810786
ACTGTCC14700.015.6130548
TATACAC225350.014.96530145
TATACTG38250.014.8823245
GTCTATG29600.014.4300221
ACTGAGC29800.014.4218898
CTAGACC18750.014.2799734
ACTGCTC22250.013.955788
CTAGACG9700.013.9174994
GACGGTC15050.013.9043767
ATACACA158750.013.85086545
AGTACCG23450.013.8166055
TACCGTG20500.013.6104627
TAAGGTG29500.013.5762465
GACTGTG8500.013.50077
CTAGACA34100.013.328424
GACAGTG11700.013.269927
Produced by FastQC (version 0.11.2)