FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l02n02 heat 2h 3.34200000008239.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l02n02 heat 2h 3.34200000008239.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15446346
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG11850.023.1649075
GACGGTC10800.019.5841377
CCGTGCG8200.018.3859149
TGCGTCG11400.018.16317225
GTCTAGG33550.017.2191541
GTCGTGC5500.017.1825248
CCTAGAC27750.017.0235313
TGACGCT10100.016.91658445
GCGTCGT12100.016.74219526
GTACCGT16200.016.666926
GTCGCGC10300.016.38613124
TATACAC256150.016.33324445
GACTGTG8950.016.0900467
GAGCGCG15400.016.0720887
AGCGCGC13800.015.9789178
GTCTATG33650.015.8319531
GTGTGAG14000.014.932281
ACCGACG6650.014.8878298
ATACACA163850.014.77711745
AGACCGA17600.014.7019276
Produced by FastQC (version 0.11.2)