FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l02n02 heat 2h 1.3420000000821f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l02n02 heat 2h 1.3420000000821f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17989658
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG12100.019.8963095
TGACGCT13750.019.62260445
GACGGTC10150.018.1778477
CCGTGCG10950.018.0839549
GAGCGCG18000.017.3754847
TATACAC317400.017.1784145
GTACCGT19600.016.759886
CCTAGAC32400.016.7328073
TGCGTCG14500.016.45193925
AGCGCGC16000.016.1723258
GCGTCGT14900.016.16284626
GACTGTG9350.016.1234447
GTCTATG36550.015.3772981
GTCGCGC13550.015.27909224
GACAGTG12500.015.1204217
AGTACCG30800.015.0484095
GTCTAGG36000.014.5505971
GTGTTAC9450.014.5119391
TACCGTG26650.014.184267
AGACCGA18850.013.9652466
Produced by FastQC (version 0.11.2)