FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l02n02 drought recovery 4h 2.342000000093ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l02n02 drought recovery 4h 2.342000000093ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12895329
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG9300.020.0810285
GTACCGT15800.017.516166
GACGGTC10850.015.7614667
GTCTAGG23650.015.6824481
CCTAGAC23800.015.5949813
GACTGTG7400.015.5078377
AGTACCG22050.015.102335
ACTGTCC13000.014.1932968
TATACAC223400.014.13865645
GACAGTG10350.014.1314197
TACCGTG20000.014.0634787
GCCCTAG11850.014.0370041
ATACACA156900.013.98433645
TATACTG38400.013.9455795
GTCTATG30400.013.6052121
GGACCGT18100.013.3013616
ACTGTCA6300.013.20356844
CTAGACC18600.012.9437954
TACACAT100450.012.82388645
GAGTGTC17400.012.8026167
Produced by FastQC (version 0.11.2)