FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l02n02 drought 2h 2.342000000081da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l02n02 drought 2h 2.342000000081da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15194653
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG11200.020.6923245
GACGGTC10900.020.2301757
CCGTGCG8750.020.0593419
GTACCGT17700.019.1952486
TGACGCT9500.019.17090245
TGCGTCG12350.017.67627325
GACTGTG7200.017.5007087
GTCGCGC13650.016.98044224
GGACTGC23650.016.4590596
GTCGTGC4800.016.4069148
GAGCGCG17150.016.2688797
AGTACCG26200.016.2311975
GCGTCGT13300.016.07631526
GTCTATG33250.016.0246751
TATACAC222500.015.673388545
GTCTAGG30050.015.6363111
GCCTAGT6350.015.2239481
GTGCGCT9550.015.07988911
GGTCCGT19650.015.00134811
AGCGCGC15600.015.0006068
Produced by FastQC (version 0.11.2)