FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l02n02 control 4h 1.34200000009378.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l02n02 control 4h 1.34200000009378.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14759527
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG12700.021.437155
GACGGTC11250.019.6015937
CCGTGCG9700.018.0949299
GTCGCGC13300.016.75107424
GTCTAGG34550.016.7218071
CCTAGAC30800.016.652513
GTACCGT18400.016.508266
GAGCGCG16950.015.7977447
TGCGTCG12450.015.72721125
GCGTCGT12700.015.59626126
TGACGCT9800.015.3699345
GTCTATG35000.015.2864661
CGTCGCG14600.015.25953923
GTGCGCT9950.015.15239611
GACAGTG11750.015.1288897
AGTACCG26750.014.8879495
GCCCTAG15150.014.8383471
ACTGTCC16800.014.5994018
ACGACTA8250.014.44440242
AGACCGA16450.014.3617986
Produced by FastQC (version 0.11.2)