FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l02n02 control 2h 1.34200000008197.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l02n02 control 2h 1.34200000008197.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17435698
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTCG13850.018.8497425
AGCGCGC16700.017.9194668
TGACGCT10800.017.9022745
TAGACGG12900.017.7901845
GCGTCGT15100.017.73896626
GAGCGCG18500.017.6354337
GTACCGT17650.017.5915586
CCGTGCG8800.017.3881579
GACGGTC11150.017.1527587
GACTGTG8350.016.7068717
GTCGCGC13950.016.29298624
GGACTGC26000.015.9226166
GTCTAGG33450.015.7256381
CCTAGAC31350.015.5706263
TATACAC279500.015.47592645
GTCTATG37250.015.4490651
TACCGTG24350.014.877057
GTGTATG47000.014.5878251
ACGACTA9250.014.3416342
ATACACA190750.014.24935345
Produced by FastQC (version 0.11.2)