FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l02n01 root 2.3410000000942a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l02n01 root 2.3410000000942a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19457105
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG4450.016.6862965
CTAGACT5450.014.8631894
TATACCG10250.014.4885885
ATACACA843200.014.43804345
TATACAC1225250.014.15397545
TACACAT572600.013.528419545
TACACCG51300.013.3779175
ACACATC411550.013.37196445
CTCCGAG79200.012.78346845
TAGGACA9950.012.437864
TCCGAGC69800.012.37762145
TTATACT3651.8189894E-1212.3294354
TCTCCGA108400.012.32873545
CACATCT297650.012.298243545
TACACAG8300.012.1994565
CCCTTAG6000.012.0125361
ACTGTGT7350.011.9383178
ACTGTGC19100.011.7796538
TACACTG8450.011.7166115
TCTAAAC6050.011.529553
Produced by FastQC (version 0.11.2)