FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l02n01 heat recovery 4h 2.341000000092f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l02n01 heat recovery 4h 2.341000000092f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14320515
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG11200.020.894465
GACGGTC10550.018.7670527
GTACCGT14600.017.8780866
GTCTAGG26600.016.76581
TATACAC319850.016.1224545
AGTACCG22500.015.6011975
CCTAGAC28850.015.4431273
TACCGTG19050.015.2356367
GTCGTGC5850.014.9994248
GACAGTG9950.014.69792657
ATACACA210650.014.53648945
CCGTGCG10150.014.1875389
GGACCGT17450.014.1844716
GCCTAGT6550.014.0988361
GGACTGC21600.014.06358056
ACTGTCC13900.013.7584658
GTATAAG48800.013.6157921
GTCTATG28550.013.5694751
TAAGGTG30800.013.3695345
GAGTGTC18850.013.3681877
Produced by FastQC (version 0.11.2)