FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l02n01 heat 4h 3.341000000093be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l02n01 heat 4h 3.341000000093be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11309375
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG9800.024.7976515
GACGGTC10700.018.7140257
CCTAGAC22950.017.4521983
GTCTATG24550.016.6975041
GTACCGT12450.016.446936
GACAGTG10300.016.3826837
ACTGTCC11700.015.576158
AGTACCG20150.015.1871635
ACTGCTC18300.015.1222018
CTAGACA31450.014.5956134
TATACAC138100.014.38560145
AGACGGT15350.014.2192336
AGACACT17000.014.1627536
AACAGAC14250.014.0519357
CACTGAG30200.013.7824287
ACGGTCC14700.013.7748278
GTGTGAG11400.013.6325121
GACTGTG6950.013.5964487
GTCTAGG22250.013.5645821
TATACTG32150.013.5079415
Produced by FastQC (version 0.11.2)