FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l02n01 heat 30 min 3.34100000009338.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l02n01 heat 30 min 3.34100000009338.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13965125
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG10850.020.5312825
GTACCGT17750.015.8460946
ACTGTCC13700.015.4375728
CCTAGAC26750.014.8888133
TATACAC226900.014.86362845
GTCTAGG27550.014.7138771
GGACTGC23350.014.4548956
AGTACCG25050.014.3721865
ATACACA157550.014.02331845
TATACTG35600.013.8421875
GACGGTC15300.013.8231857
CTAGACA32950.013.79451754
GCCCTAG13400.013.613081
GCCTAGT6850.013.4793661
AACAGAC17000.013.3673147
TACCGTG21450.013.3213467
ACGGTCC17850.013.2349638
ACTGCTC23000.013.1083698
TCTAGAC29800.012.9874163
GTATAAG47450.012.9094841
Produced by FastQC (version 0.11.2)