FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l02n01 heat 2h 3.3410000000823c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l02n01 heat 2h 3.3410000000823c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15446346
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG12250.023.6950575
GACGGTC11350.021.408687
GTACCGT17350.019.7127976
GCGTCGT13050.018.27493726
TGCGTCG13150.018.13596325
GTCGCGC12950.017.72116924
CCGTGCG7850.017.4842119
CCTAGAC27800.017.4019453
AGCGCGC15300.017.0580168
GAGCGCG17700.016.905987
AGTACCG27450.016.5582035
TATACAC254700.016.43936345
CGTCGCG14750.015.86365623
TACCGTG23050.015.4222637
ACTGTCC15050.015.3979428
GTCGTGC5700.015.3940098
GTCTAGG30600.015.0143571
AACAGAC16250.014.3993187
ATACACA169150.014.36545445
ATAGACG19450.014.22954
Produced by FastQC (version 0.11.2)