FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l02n01 heat 2h 1.34100000008212.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l02n01 heat 2h 1.34100000008212.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17989658
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG13350.022.7545575
GACGGTC12600.019.999377
CCGTGCG9700.018.788859
TGCGTCG13600.018.36308525
GTCGCGC14300.017.77886424
GCGTCGT14300.017.77886426
GAGCGCG17350.017.5066537
TATACAC316000.017.28002245
GTACCGT20650.017.216826
GACTGTG9700.017.1644087
CCTAGAC33200.017.1474023
AGACCGA21650.016.7333896
AGCGCGC15800.016.5184678
ACTGTCA8900.015.92646644
GACAGTG10800.015.8328347
AAGACCG31500.015.286895
GTCGTGC5600.015.2673778
GGACTGC27450.014.8372076
GTCTATG37300.014.73216151
GTCTAGG36850.014.606491
Produced by FastQC (version 0.11.2)