FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l02n01 drought recovery 4h 2.341000000093f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l02n01 drought recovery 4h 2.341000000093f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12895329
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG9200.018.833175
CCTAGAC25100.017.660913
GACAGTG10100.016.9304037
GTACCGT15550.016.06256
GTCTAGG23500.015.9086441
GACTGTG7500.014.99974257
CTAGACA30850.014.9526864
AACAGAC14650.014.7437747
GACGGTC10200.014.337997
CCGTGCG11150.014.3276639
TATACAC222500.014.23751645
TATACCG38750.014.0528435
GGACCGT17400.013.966746
ATACACA159650.013.90944545
TACCGTG19650.013.8547247
CCCTAAG18050.013.7248741
ACTGCTC20000.013.61226758
ACTGTCC13600.013.5659438
ACCGTGC25900.013.465028
AGTACCG22300.013.4204585
Produced by FastQC (version 0.11.2)